Also, the 3-year old plasmid stored at −20 ☌ and the AAC-tested plasmid stored up to 65 ☌ for 20 days had functional integrity and nucleotide integrity comparable to control sample, thereby allowing for retrieval of the original error-free text data.

The plasmid DNA under accelerated aging conditions (AAC) up to 65 ☌ for 20 days, which corresponds to approximately 20 years of storage at −20 ☌, also exhibited no significant differences in DNA stability compared to newly produced plasmid. A long-term DNA stability study demonstrated that 3-year stored plasmid containing text information showed DNA stability at controlled conditions of −20 ☌. The DNA comprised a total of 22 chemically synthesized DNA fragments with 400 nucleotides each, which were incorporated into a plasmid vector. A document consisting of 2046 words was encoded with DNA sequences using Perl script, and the encoded DNA sequences were synthesized for information storage. Because of this, we evaluated the plasmid-based DNA data storage in a manner that preserves DNA stability and integrity. Validation of long-term DNA stability and integrity are essential for the use of DNA in data storage applications.

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